Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: Expression of B7-H3 target in osteosarcoma samples and tumor cell lines. A B7-H3 expression was detected in OS tissues. OS Osteosarcoma. scale bar = 50 μm. B The surface expression of B7-H3 receptor level on tumor cell lines (Jurkat, HOS, U-2 OS, SW1353, Saos-2) detected by flow cytometry

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: Expressing, Flow Cytometry

Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: Construction of 3rd-generation CAR-T cells targeting B7-H3. A Schematic diagram of the B7-H3 CAR-T transgene. B Proportion of B7-H3 CAR infected primary human T cells determined by flow cytometry. C Histogram of the B7-H3-CAR-T rate of B7-H3 CAR infected cells and its control cells in three repeats. (mean ± SEM; ns not significant P > 0.05, * P < 0.05,** P < 0.01, *** P < 0.001)

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: Infection, Flow Cytometry

Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: Cell phenotype of B7-H3 CAR-T cell. A Flow cytometry to detect CD3 + /CD4 + /CD8 + /CD45RA + /CCR7 + ratio in CAR-T cells and NC-T cells B Detection exhaustion biomarker of TIM3, PD1, LAG3 on CAR-T cells and NC-T cells by Flow cytometry.

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: Flow Cytometry, Biomarker Assay

Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: Functional Study of B7-H3 CAR-T cells in vitro. A The cytotoxicity of B7-H3 targeted CAR-T cells against solid tumor cell lines was analyzed by RTCA assay. The red arrow represents the time point of effector cell addition. B Comparison of killing rates under different E:T ratios on tumor cells. C Levels of IFNγ and TNFα released by B7-H3 CAR-T cells analyzed by ELISA after incubated with cells for 20 h. D Situation of effector cells and target cells (U-2 OS) displayed by optical microscopy. mean ± SEM; ns not significant P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: Functional Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Incubation, Microscopy

Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: Anti-tumor efficacy of B7-H3 CAR-T cells against PDX models of osteosarcoma in vivo . A and G Schema of the experimental events and nodes. The tumor size (B and H) and mice weight ( F and J) variation with B7-H3 CAR-T cells and control T cells injection among the Observed time. C Presence of CD3 + T cells in mouse peripheral blood after 10 days of injection. D Absence of CAR-T cells in mice tumors after 10 days of injection. E Size of tumor mass differed in B7-H3 CAR-T cells, NC T cells or PBS treated mice. I Kaplan–Meier curves for survival of the PDX-OSTE0007 models are shown. (mean ± SEM; ns not significant P > 0.05, * P < 0.05,** P < 0.01, *** P < 0.001)

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: In Vivo, Injection

Journal: BMC Cancer

Article Title: B7-H3 targeted CAR-T cells show highly efficient anti-tumor function against osteosarcoma both in vitro and in vivo

doi: 10.1186/s12885-022-10229-8

Figure Lengend Snippet: B7-H3 positivity rate in OS samples

Article Snippet: Recombinant B7-H3 FITC conjugated antibody (Sino Biological Inc., Beijing, China) was used to detect the expression of B7-H3 protein on cancer cells by flow cytometry.

Techniques: